8. Urine samples can miss up to 10% of underlying infections where vaginal samples miss fewer than 1% of infections. Do not touch the soft tip or lay the swab down. While holding the swab in the same hand, unscrew the cap from the tube. b. The correct volume of urine has been added when the fluid level is between black fill lines on urine specimen transport tube label. Methodology. Room temperature: 60 days; Refrigerated . Re-cap urine specimen transport tube tightly. Partially peel open the swab package. lines on the urine specimen transport tube label. ‡. Transfer 1 mL of specimen into the Aptima Specimen Transfer Tube using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug). While holding swab in hand, unscrew tube cap. Transfer specimen into the Aptima Specimen Transfer Tube within 24 hours of collection. 8. If tube contents are spilled, discard and replace with a new Aptima Multitest Swab Specimen Collection Kit. Transport Aptima Specimen Transfer Tube (refrigerated is preferred) within 30 days of collection. Ship to lab. Transport Temperature. Transfer 0.5 mL . Immediately place swab into transport tube so black score line is at top of tube. RNA Amplification Hologic APTIMA® . c. Recap Aptima Specimen Transfer Tube tightly and gently invert 3 times to mix. • Transfer urine sample into Aptima urine specimen transport tube within 24 hours of collection. Swab, Aptima Multitest Swab Specimen Collection Kit (T584) Specimen Type: Ocular . Container/Tube: Aptima Collection Multitest Swab or Aptima Swab Collection System. Immediately place . These swabs are contained in the APTIMA Collection Kit. 4. Intended Use The Aptima® SARS-CoV-2 assay is a nucleic acid amplification US - English. Recap the transfer tube tightly and invert gently 2 to 3 times to ensure complete mixture of sample. When collecting multiple specimens from the same patient, the tube label provides a specimen source field for unique identification for specimen location. Do not spill tube contents. Liquid level must be between fill lines on tube. Once an aliquot of the PreservCyt Solution in the ThinPrep vial has been transferred to the APTIMA Specimen Transfer Tube, the specimen is stable for 14 days from the date of transfer if stored refrigerated. If the tube contents are spilled, discard and replace with a new Aptima unisex swab transport tube. File Size: 102KB Page Count: 1. Remove the cap of the APTIMA urine specimen transport tube. Hologic APTIMA Urine Specimen Collection Kit (yellow label tube) *NOTE: Male Anal and Throat sites, use orange label Multitest Swab as above Male - a first void urine passed into urine pottle (first 20mL of stream, preferably at least 1 hour after last voiding) Remove cap from yellow labelled tube. It is also the most frequent cause of non-gonococcal urethritis in men. . Transfer 1 mL of specimen into the Aptima Specimen Transfer Tube using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug). Discard pipette. A vaginal specimen is required if the . 1 Correct! Do not spill tube contents. It is also the most frequent cause of non-gonococcal urethritis in men. Discard top . Put on clean gloves before proceeding to specimen transfer. 002. transferred into the APTIMA Specimen Transfer Kit for testing with the APTIMA Combo 2 Assay. The Aptima ® Multitest Swab detects up to 7 infections or disease states at a time. Swab Specimens: The multitest (vaginal) or unisex swab specimen will be stored at room temperature for 14 days from collection. Carefully break the swab shaft at the score line against the side of the tube. This is now known as the "processed urine specimen." Patient should not have urinated for at least 1 hour prior to specimen collection. Align the . Refer to the appropriate Aptima assay package insert for additional storage and handling information. The vaginal swab specimen The vaginal swab specimen . If the lab receives a swab specimen transport tube with no swab, two swabs, a cleaning swab, or a swab not supplied by Hologic the specimen must be rejected. Hologic Aptima Transfer Tube with Blue swab: Refrigerate: Chlamydia/GC (Urine) Hologic Aptima Urine Specimen Collection Kit: Refrigerate: Ear/Eye Culture: Amies culturette (blue top) Room Temperature: Fungal Culture: Must state source; any culturette; hair, skin etc in clean cup: Room Temperature: While holding swab in hand, unscrew tube cap. Store at 2°C to 30°C and test within 30 days of collection. Aptima Specimen Aliquot Tubes (SAT) are intended for use with plasma and serum specimens that will be tested with Aptima® quantitative assays. The Direct Load Tube Collection Kit is authorized for use with the Aptima® SARS-CoV-2 and Aptima® SARS-CoV-2/Flu assays. Transport Container. Carefully break the swab shaft at the score line against the side of the tube. Tightly screw cap onto tube. 5. If tube contents are spilled, discard and replace with a new Aptima unisex swab transport tube. 3. Process only 1 ThinPrep and transfer tube set at a time. 009 General Information Aptima® SARS-CoV-2 . After transfer to APTIMA specimen transfer tube, store at 15-30°C for 14 days or store at 2-8°C for 30 days. Follow instructions in the APTIMA® Specimen Collection Kits for Vaginal, Endocervical and Urethral Swab Specimens package insert. C. trachomatis is known to cause cervicitis, pelvic inflammatory disease (PID), epididymitis and proctitis. Remove the cap of the APTIMA urine specimen transport tube. Uncap the PreservCyt vial and place the cap on the bench with the threads facing up. This is now known as the "processed urine specimen." If contents of tube spill, use a new Hologic® APTIMA® Vaginal Swab Specimen Collection Kit. 9. 7. Immediately place swab into transport tube so black score line is at top of tube. 7-10. • Store between 2˚C to 30˚C and test within 30 days of collection. Specimen Stability. Remove cap and transfer 2 mL of urine into urine specimen transport tube using the pipette provided. 1. A vaginal sample provides more accuracy than a urine sample. Aptima SARS-CoV-2 - Panther System 2 AW-21492 -001 Rev. c. Recap Aptima Specimen Transfer Tube tightly and gently invert 3 times to mix. diagnostic.solutions@hologic.com. Collection Info. If the soft tip is touched, the swab is . Cap tube securely and label tube with patient's entire name and collection date and time. • After collection, transport and store swab in unisex specimen transport tube between 2˚C to 30˚C until . Chlamydia trachomatis RNA, TMA, Urogenital - C. trachomatis infections are the leading cause of sexually transmitted diseases in the united states. Transfer 2 mL of urine from the collection cup to the . Re-cap the urine specimen transport tube tightly. Specimens in the Aptima Multitest tube may be stored at 2°C to 30°C up to 6 days. From the ThinPrep system main menu, select "4-GYN" by pressing 4 on the keypad. If tube contents are spilled, discard and replace with a new Aptima Multitest Swab Specimen Collection Kit. 502260-EN-RevB.pdf. Uncap the PreservCyt vial and place the cap on the bench with the threads facing up. 3. must be used for specimen transfer. Do not spill tube contents. Do not spill contents of tube. General Information . 7. VTM lesion swab specimens transferred to the Aptima Specimen Transfer tube must be tested with the Aptima HSV 1 Remove the cap of the APTIMA® Urine Specimen Collection tube and transfer 2 mL of urine into the urine specimen transport tube using the disposable pipette provided. Do not spill tube contents. One mL of peritoneal fluid was added to a Hologic APTIMA specimen transfer kit then spiked with C trachomatis and Neisseria gonorrhoeae at the limit of detection. Transfer 2 mL of urine from the collection cup to the . If the lab receives a swab specimen transport tube with no swab, two swabs, a cleaning swab, or a swab not supplied by Gen-Probe, the specimen must be rejected. Loosen the cap on the Aptima® tube. This is now known as the processed urine specimen. transfer tube. This is now known as the "processed urine specimen." Specimen Transport and Storage 1. Do not spill tube contents. Hologic, Inc. 10210 Genetic Center Drive . c. Recap Aptima Specimen Transfer Tube tightly and gently invert 3 times to mix. Specimens are transferred to a tube containing specimen transport media (STM) that lyses the cells, releases the mRNA, and protects it from degradation during storage. Synonyms. Withdraw swab without touching skin. Unisex Swab: Place blue swab in Swab Specimen Transport Tube, break shaft off at the scoreline, then recap tube. Among women, the consequences of chlamydial infections are severe if . Days Performed: Monday through Friday. Specimen Transport and Storage After collection, transport the processed urine specimens in the APTIMA urine specimen transport tube at 2°C to 30°C and store at 2°C to 30°C until tested. 301041. Immediately place swab in transport tube so that tip of swab is visible below tube label. Container/Tube: APTIMA Unisex Swab for Endocervical Specimens. While holding swab in hand, unscrew tube cap. Recap the swab specimen transfer tube tightly, label and ship to the lab. Discard top portion of swab shaft. Store tubes at room temperature 2. . Swab site using Aptima . Transform specimen processing for COVID testing. level is between black fill lines on urine specimen transport tube label. Within 1 minute of vortexing, transfer 1 mL of the PreservCyt specimen into the Aptima Specimen Transfer Tube. Re-cap swab specimen transport tube tightly. Submit swab in the provided transport tube/media contained within the Aptima® Swab Specimen Collection Kit. Hologic Aptima ® Collection Kit supplied must be used to collect patient specimens for this test. Room temperature. Withdraw swab without touching the skin. • APTIMA® Specimen Transfer Tube: 14 days containing SurePath™ aliquot, 30 days containing ThinPrep® aliquot. During specimen collection, avoid contact between the swab and lubricant. 4 AW-15641-REG Rev. Container/ Tube: Hologic APTIMA Unisex Swab or Hologic APTIMA Urine transport tube supplied in the Hologic APTIA Collection Kits Type: Female endocervical swab or female urine specimens, collected using Hologic APTIMA Specimen Collection Kits Volume: Inoculated swab in 2.9 mL of transport media or 2 mL urine added to 2.0 mL of transport media . Insert the specimen collection swab (blue shaft swab in the package with the green printing) 2 to 4 cm into the urethra. 9. Withdraw the swab carefully without touching your skin. If longer Vaginal swab specimens are as sensitive as cervical swab specimens and there is no difference in specificity. 1 " - CDC. Vaginal swab : Use the APTIMA Vaginal Swab Specimen Collection Kit (orange label on Vaginal Swab Transport Media tube) 1. Label each Aptima® tube with the appropriate specimen label. Chlamydia trachomatis (CT), Neisseria gonorrhoeae (GC) and Trichomonas vaginalis (TV) can all be tested from the one APTIMA® Multitest Swab Specimen Collection Kit. After collection, transport and store the processed urine specimens in the APTIMA urine specimen transport tube at 2°C to 30°C until . Once an aliquot of the PreservCyt Solution in the ThinPrep vial has been transferred to the APTIMA Specimen Transfer Tube, the specimen is stable for 14 days from the date of transfer if stored . Load the ThinPrep Pap Test vial into the ThinPrep 2000 System. Aptima Urine Specimen Collection Kit for Male and Female Urine Specimens. Specimen Transported Temperature: at 2 to 30 °C Submit only 1 of the following specimens: Hologic Aptima ® Collection Kit supplied must be used to collect patient specimens for this test. Align the score . Aptima® VTM Lesion Swab Specimen Procedural Notes Aptima Specimen Transfer Kit 5 AW-15640 Rev. Collection Instructions: 1. 1. Obtain specimen using a APTIMA® swab specimen transport tube. If tube contents are spilled, discard and replace with a new Aptima Multitest Swab Specimen Collection Kit. a. Currently, the "gold standard" involves the use of quantitative real-time polymerase chain reaction the tube tightly. Chlamydia/Neisseria gonorrhoeae RNA, TMA, Urogenital - C. trachomatis infections are the leading cause of sexually transmitted diseases in the United States. Aptima Specimen Transfer Tube workflow for use with the assay and supporting analytical and clinical validation, (2) add the Aptima Unisex Swab . While holding swab in hand, unscrew tube cap. Process only 1 ThinPrep and transfer tube set at a time. 6. a. Carefully break the swab shaft at the score-line; use care to avoid splashing of the contents. Withdraw swab without touching skin. b. Discard top portion of swab shaft. O. Do not spill the contents of the tube. Vortex the PreservCyt vial for 3 to 10 seconds. Specimen Type(s) Swab, Tissue. 301162. 6. While holding the swab in hand, unscrew the tube cap. If the lab receives a swab specimen transport tube with no swab, two swabs, a cleaning swab, or a swab not supplied by Hologic the specimen must be rejected. Break at b. Carefully break swab shaft against side of tube. If shipping temperatures exceeding 30°C are anticipated, use cool plack. 2. US - Englishi. Tightly screw cap onto tube. Obtain specimen using a APTIMA® swab specimen transport tube. Do not spill tube contents. No uncapping and no specimen transfer. Recap the transfer tube tightly and invert gently 2 to 3 times to ensure complete mixture of sample. Remove excess mucus with cleanning swab (White shaft) and discard. Aptima Multitest Swab Specimen Collection Kit. In females, to ensure collection of an adequate specimen, columnar epithelial cells lining the endocervix should be . Proprietary Name: Aptima Cervical Specimen Collection and Transport Kit; Aptima Multitest Specimen Collection Kit; Aptima Specimen Transfer Kit; Aptima Unisex Swab Specimen Collection Kit; Aptima Urine Specimen Collection Kit; Aptima Urine Specimen Transport Tubes; Hologic Direct Load Capture Cap Collection Kit - CLASSIQSwabs; Hologic Direct Load Capture Cap Collection Kit - FLOQSwabs; Hologic . 3. Follow the instructions below for the collection of nasopharyngeal (NP) specimens using th e APTIMA Unisex Swab Specimen Collection Kit COLLECTION USING APTIMA UNISEX COLLECTION KIT- see page two for photo . SATs are included in the Aptima® Specimen Diluent Kit. Endocervix (Females Only) 1. While holding swab in hand, unscrew the tube cap. P. In the specimen transfer area, place a test tube rack containing a sufficient number of Aptima® tubes to the number of ThinPrep® Pap specimens being tested for Ct/Ng. The correct volume of urine has been added when the fluid level is between the black lines within the clear pane on the urine specimen transport tube label. Chlamydia Aptima swab: Hologic Aptima Transfer Tube with Blue swab: Refrigerate: Chlamydia/GC (Urine) Hologic Aptima Urine Specimen Collection Kit: Refrigerate: Ear/Eye Culture: Amies culturette (blue top) Room Temperature: Fungal Culture: Must state source; any culturette; hair, skin etc in clean cup: After collection, transport the processed urine specimens in the APTIMA urine specimen transport tube at 20C to 30'C and Specimen store at 20C to 30'C until tested . Aptima ® Throat Swab Specimens Collection and Handling. PreservCyt®: Transfer 1 mL of PreservCyt® solution into APTIMA® Vaginal Collection tube or Multi-test Collection Tube (orange label), or APTIMA® Specimen Transfer tube (green label). and patient-collected vaginal swab specimens use only the APTIMA . a. The PreservCyt liquid Pap specimens and endocervical swab specimens were tested with the APTIMA Combo 2 . Vaginal Swab: Place pink swab in Swab Specimen Transport Tube, break shaft off at scoreline, then recap tube. 4. SurePath™: SurePath™ fluid must be transferred to Aptima® tube within 4 days of collection. Specimen Volume: Swab. APTIMA Urine Collection Kit for Male and Female Urine Specimens (APTIMA Combo . Specimen Lysis Tubes and Specimen Transport Medium (STM) are intended to be used for processing specimens for use with Hologic assays. 2. Collection Kit / Aptima Specimen Transport Tubes, Aptima Cervical Specimen Collection and Transport Kit Recommended Use In vitro diagnostic Details of the supplier of the safety data sheet United States of America SupplierHologic, Inc. 10210 Genetic Center Drive San Diego, CA 92121-4362 Canadian Supplier Hologic Canada Limited 130 Spadina . If the tube contents are spilled, discard and replace with a new Aptima vaginal swab specimen collection kit. Remove cap from Urine Specimen Transport Tube and, using the disposable . Once an aliquot of the PreservCyt Solution in the ThinPrep vial has been transferred to the APTIMA Specimen Transfer Tube, the specimen is stable for 14 days from the date of transfer if stored refrigerated. Page Count: 1 . Transfer the urine sample into the Aptima urine specimen transport tube within 24 hours of collection. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Screw cap on securely. "Specimens obtained with a vaginal swab are the preferred specimen type. Prior to rejecting a swab transport tube with no swab, verify that it is not an APTIMA Specimen Transfer Tube as this specimen transport tube will not contain a swab. Alternatively, submit the APTIMA® unisex swab transport with white label for amplified detection of Ct, Ng, Tv, M hominis, U urealyticum, M genitalium, or HSV 1 & 2 from endocervical or male urethral specimens. Package Inserts; Safety Data Sheets; Main navigation (Footer) Women's Health Products 004 8. Swab Specimens: The multitest (vaginal) or unisex swab specimen will be stored at room temperature for 14 days from collection. • Urine samples still in primary collection container must be transported to the lab between 2˚C to 30˚C. 5. 3. If the contents of the tube are spilled, use a new Aptima Multitest Swab Specimen Collection Kit. Do not spill tube contents. Align the score line with top edge of tube and carefully break shaft. Re-cap the swab specimen transport tube tightly, label and ship to the lab. Aptima® Multitest Swab Specimen Collection Kit Clinician collection procedure guide For rectal swab specimens Carefully insert swab into the rectum about 1-2 inches (3-5 cm) past the anal margin (the outside of the anus) and gently rotate swab clockwise for 5-10 seconds. Re-capurine specimen transport tube tightly. Forms. Endocervix (Females Only) 1. APTIMA® transport tube. Make sure swab touches the vagina walls so that moisture is absorbed by the swab. Container/Tube: APTIMA Unisex Swab for Endocervical Specimens. 3. Carefully break the swab shaft at the score line against the side of the tube. ThinPrep vial: Specimens submitted in the ThinPrep liquid Pap vial must be transferred to an APTIMA Specimen Transfer Tube within 30 days of collection. PreservCyt®: Transfer 1 mL PreservCyt® solution into Aptima® Specimen Transfer Tube (green label) or Aptima® Vaginal Collection or Multitest Collection Tube (orange label) or Unisex Swab Specimen Collection Kit (white label). . Aptima Unisex Specimen Collection Kit for Endocervical and Male Urethral Swab Specimens. If longer . Support / Safety Data Sheets Support Menu: Hidden. 2. Specific use(s) Aptima Vaginal Swab Collection Kit / Aptima Multitest Swab Specimen Collection Kit, Aptima Specimen Transfer Kit / Aptima Liquid Pap Transfer Tubes, Aptima Unisex Collection Kit / Aptima Specimen Transport Tubes, Aptima Cervical Specimen Collection and Transport Kit Recommended Use In vitro diagnostic Details of the supplier of . swab specimen transport medium and tube within 72 hours of collection Store at room temperature Source images courtesy of HOLOGIC, Inc, Aptima® Multitest Swab Specimen Collection Kit Clinician Collection Procedure Guide for Throat Swab Specimens DS-09496-001 Rev.003 please contact your local Hologic representative or write to diagnostic.solutions@hologic.com. If tube contents are spilled, request a new Aptima Multitest Swab Specimen Collection Kit. Carefully insert swab into the vagina about 2 inches (5 cm) past the introitus and gently rotate swab for 10 to 30 seconds. Within 1 minute of vortexing, transfer 1 mL of the PreservCyt specimen into the Aptima Specimen Transfer Tube. Insert colelction swab (Blue shaft) and rotate for 15 to 30 . Vaginal swab specimens must be transported to Alberta Precision Labs in the provided swab specimen transport medium and tube within 72 hours of collection Store at room temperature Source images courtesy of HOLOGIC, Inc, Aptima® Multitest Swab Specimen Collection Kit Clinician Collection Procedure Guide for Vaginal Swab Specimens DS-01552-001 . Viability after transfer to collection/transport tube is: • ThinPrep® Pap Vial: 30 days. Fill out the requisition provided for COVID -19 or enter into your EMR and label the tube with: *1-2,3. 502258-EN-RevB.pdf. Immediately place swab into transport tube so the black score line is at the top of the tube. With just one sample, the Aptima Multitest Swab simplifies testing and provides more answers for sexual and vaginal health. Using the same swab, carefully insert the swab into the second nostril until . C. trachomatis is known to cause cervicitis, pelvic inflammatory disease (PID), epididymitis and proctitis. Remove the swab. Transfer 1 mL of specimen into the Aptima Specimen Transfer Tube using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug). All pipettors must be . After transfer to an Aptima specimen transfer tube, specimens must be stored at 2°C to 30°C until tested. Expedite your SARS-CoV-2* workflow with a collection device that loads directly on the Panther® system. Urine: Urine must be placed in an Aptima Urine Specimen Transport Tube within 24 hours of collection. If the lab receives a swab specimen transport tube with no swab, . If tube contents are spilled, discard and replace with a new Aptima unisex swab transport tube. Carefully insert swab into opening of the vagina, about 2 inches (5 cm), and gently rotate swab for 10 to 30 seconds. Container/ Tube: Hologic APTIMA Unisex Swab or Hologic APTIMA Urine transport tube supplied in the Hologic APTIA Collection Kits Type: Female endocervical swab, male urethral swab, female and male urine, throat or rectal swab specimens, collected using Hologic APTIMA Specimen Collection Kits Volume: Inoculated swab in 2.9 mL of transport media or 2 mL urine added to 2.0 mL of transport media True False. Process only 1 ThinPrep and transfer tube set at a time. The correct volume of urine has been added to the Aptima tube when the fluid level is between the black fill lines on the transport tube. Keep tube upright - tube contains liquid . the swab a few times against the nasal wall and remove from nostril. When the APTIMA HPV 16 18/45 Genotype Assay is performed, the target mRNA is isolated from the specimen by Swab: Place swab in Swab Specimen Transport Tube, break shaft off at scoreline then recap tube. . File Size: 102KB. the swab into transport tube and carefully break the swab shaft against the side of the tube. Fill between black lines only. Make sure swab touches vaginal walls so . Aptima Multitest Swab Specimen processing guide Aptima Urine Specimen collection procedure Product: A03 Supply # 180028 Product: A01 Supply # 180029 Product: A02 Supply # 180032 Product: A03 Supply # 180028 Product: A01 Supply # 180029 Product: A02 Supply # 180032 Accept Reject Transfer urine into collection tube. Swabs: Swab must be submitted in APTIMA® Vaginal Swab Specimen Collection kit or Multi-Test collection kit, or Unisex Swab Specimen Collection tube. The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. 6. 301040. Among women, the consequences of Chlamydial infections are . Male Urethral Swab Specimens (Unisex Swab kit = white tube only) The patient should not have urinated for at least 1 hour prior to sample collection. . After transfer to APTIMA specimen transfer tube, store at 15-300C for 14 days or store at 2-80C for 30 days. Recap the swab specimen transfer tube tightly, label and ship to . Urine: Transfer 2 mL urine within 24 hours to Aptima Urine Specimen Transport Tube (ARUP supply #28908 PK/50 or #54556 PK/10) available online through eSupply using ARUP Connect ™ or contact ARUP Client Services at (800) 522-2787. • After collection, transport and store processed urine specimens in the Aptima urine specimen transport tube between 2˚C to 30˚C until tested . 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